Mansoura Journal of Environmental Sciences - Egypt JOESE
Vol.46 No. 2 / June2017
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| Kinetin and/or calcium affect growth of Phaseolus vulgaris L. plant grown under heavy metals stress / | |||||
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| تخصص البحث : | |||||
| Mansoura Journal of Environmental Sciences - Egypt JOESE / Vol.46 No. 2 - June2017 | |||||
| تاريخ تقديم البحث 09/04/2017 | |||||
| تاريخ قبول البحث 22/11/2017 | |||||
| عدد صفحات البحث 23 | |||||
| Aziza N. Moustafa | |||||
| Fardous M. Bassuony | |||||
| Samia A. Haroun | |||||
| Radwan R. Khalil - مؤلف رئيسي | |||||
| Antioxidant enzymes; Calcium; Kinetin; Lead; Nickel; Phaseolus vulgaris | |||||
| Heavy metals stress is one of the major abiotic stresses that cause environmental pollution and hence indefinite hazards to living organisms in recent decades. The current study was conducted with the goal in the mind to clarify the ameliorative role of seed presoaking in kinetin (30 ppm ) and/or calcium chloride (40 mM) in counteracting the deteriorative effect of foliar application of nickel (2.5 mM) and/or lead (0.5mM) on the growth and some metabolic activities of Phaseolus vulgaris L. plant. Nickel and/or lead treatments significantly reduced the leaves area/plant, total pigments, soluble and insoluble sugars, amino-nitrogen, catalase enzyme, total nitrogen, potassium, calcium and magnesium contents. On the other hand, proline, amino-N, MDA, total phenols, SOD, POX, nickel and lead contents were significantly increased in response to heavy metal treatments. On contrary, seed presoaking in kinetin and calcium chloride alone or in combination significantly increased the leaves area/plant, total pigments, soluble and insoluble sugars, amino-nitrogen, catalase enzyme, total nitrogen, potassium, calcium and magnesium contents. Meanwhile, proline, MDA, total phenols, SOD, POX, nickel and lead contents were significantly decreased by kinetin and calcium chloride treatments. Kinetin and/or calcium chloride were found to be the most effective in enhancing the plant tolerance towards nickel and/or lead toxicity. | |||||
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| Characterization of Two Drought-Tolerant PGPB: Azospirillum brasilense NO40 and Stenotrophomonas maltophilia / | |||||
| تخصص البحث : Basic Sciences | |||||
| Mansoura Journal of Environmental Sciences - Egypt JOESE / Vol.46 No. 2 - June2017 | |||||
| تاريخ تقديم البحث 26/11/2017 | |||||
| تاريخ قبول البحث 26/11/2017 | |||||
| عدد صفحات البحث 17 | |||||
| Samar Salama - مؤلف رئيسي | |||||
| Mohammed N. Omar | |||||
| Mohammed E. Osman | |||||
| Wedad A. Kasim | |||||
| Keywords: Azospirillum brasilense Drought PGPB Spermosphere Stenotrophomonas maltophilia |
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| Development of plant growth-promoting bacteria (PGPB) to enhance the growth and productivity of agronomic crops under normal and drought-stressed conditions can offer a rapid, low-cost and eco-friendly solution to meet the ever-increasing worldwide food demand. However, the selection of such PGPB is not necessarily straightforward. In this work, two PGPB, Azospirillum brasilense NO40 and an isolate identified (using 16S rDNA sequence analysis) as Stenotrophomonas maltophilia were tested as potential candidates for enhancing wheat tolerance to drought stress. Both were previously screened for the ability to fix atmospheric nitrogen and for tolerating concentrations of polyethylene glycol 6000 (PEG6000) up to 30%. The survival of the selected strains was tested under higher concentrations of PEG (up to 50%). Additionally, the effect of 25% PEG on the bacterial growth rate, culture pH and the bacterial plant growth promoting traits was studied. Finally, the capacity of the selected bacteria to enhance the tolerance of wheat seedlings to drought stress was studied in a sterilized spermosphere model. The two selected strains could survive concentrations of PEG up to 50%. The cultures’ pH was slightly alkaline over time and the cell densities were lower in the PEG-containing cultures. However, the selected strains were able to maintain their plant growth-promoting abilities under stress and consequently enhanced the lengths, fresh and dry weights and the relative water contents of wheat seedlings. Therefore, screening bacteria for tolerance to high PEG concentrations and desirable plant growth promotion traits can provide PGPB that should facilitate agricultural productivity under limited water supply. |
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| Evaluation of heavy metal pollution in Damietta Branch of Nile River, Egypt using metal indices and phyto-accumulators / | |||||
| تخصص البحث : Basic Sciences | |||||
| Mansoura Journal of Environmental Sciences - Egypt JOESE / Vol.46 No. 2 - June2017 | |||||
| تاريخ تقديم البحث 26/11/2017 | |||||
| تاريخ قبول البحث 26/11/2017 | |||||
| عدد صفحات البحث 15 | |||||
| Yasser A. El-Ameir - مؤلف رئيسي | |||||
| Keywords: Heavy metals Pollution indices Hydrophytes Phytoremediation Nile River |
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| Abstract The present study aimed to evaluate heavy metals pollution in Damietta-Nile River branch in Egypt using Heavy Metal Pollution Indices (HPIs). Eighteen water samples were collected from different sites along the Damietta branch in addition to samples of hydrophytes. The Damietta branch of Nile River could be divided into two different regions; the fresh Nile-water (El-Qanater El-Khayria-Farskour) and marine environment (Farskour-Ras El-Bar). The mean values of Fe, Mn, Zn and Pb showed the highest concentrations in water samples among the different sites, whereas Cu, Co, Cd and Ni maintained the lowest mean concentrations. The overall HPIs (367.35) was higher than the critical threshold pollution index, indicating considerable contamination of water in Damietta branch with metals that may attributed to excessive discharge of drainage water originated from different anthropogenic activities. According to the bioaccumulation factor (BAF), the heavy metal uptake by three macrophytic species followed the order Fe > Mn > Zn > Cu > Pb > Ni > Cd > Co. The hydrophytes Ceratophyllum demersum accumulated the highest metal content followed by Eichhornia crassipes and Myriophyllum spicatum. The resulted indicated that, the concentrations of the heavy metals were largely dependent on plant species and the sampling site. Finally, the obtained results revealed the important role of hydrophytes in biological treatment of polluted water in aquatic environments. |
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| Spatiotemporal Assessment of Water and Sediment Quality in Idku Lake, Egypt using Multivariate Analysis and Inverse Distance Weighting Method (GIS Tool). / | |||||
| تخصص البحث : Basic Sciences | |||||
| Mansoura Journal of Environmental Sciences - Egypt JOESE / Vol.46 No. 2 - June2017 | |||||
| تاريخ تقديم البحث 26/11/2017 | |||||
| تاريخ قبول البحث 26/11/2017 | |||||
| عدد صفحات البحث 18 | |||||
| Yasser A. El-Amier - مؤلف رئيسي | |||||
| Muhammad A. El-Alfy | |||||
| Samia A. Haroun | |||||
| Manar M. Nofal | |||||
| Keywords: Idku Lake Water Sediment Pollution GIS |
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| Abstract Egypt has natural productive water sources dispersed alongside the Northern Delta coast like Idku Lake, the point of concern of this research. It suffers either from pollution, decline in the area or decrease in its fish production, especially in the last decades. The aim of work is to identify the changes of physical and chemical composition of lake water and sediment with facility of explanation into shape of distribution maps using inverse distance weighting (IDW) method in geographical information system (GIS) program within two seasons. Geo-referenced water and sediment samples were collected from 12 sites distributed within the two basins of the lake. Samples were analyzed for scouting the different types of pollutants and distribution of it. Results showed variability between sites and seasons. Calculated average water quality index indicated good quality in winter season (45.55) and oxygenated water system when compared with summer season (143.20). Indicators specified in organic matter (Biological and chemical oxygen demand and organic matter) showed high distribution in drainage areas. Cluster analysis indicate that locations within similar under pressure conditions of drainage waters have to large extent the same physiochemical properties. The sites in northern basin of the lake also have similar results. New projects be occur now in the lake and be observed during the collection period. The lake Idku is one of the most threatened lakes in Egypt as the area was declined and water quality became bad due to different drains and fish farms that drain into it directly. Lake Idku must followed by organized plan from different authorities as; NIOF, El-Bahiera Governorate, EEAA and Fisheries Authority for maintenance its resources. |
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| Effective Microorganisms (EM) Mitigate the Effect of Wastewater on Phaseolus vulgaris L. during the Flowering Stage / | |||||
| تخصص البحث : Basic Sciences | |||||
| Mansoura Journal of Environmental Sciences - Egypt JOESE / Vol.46 No. 2 - June2017 | |||||
| تاريخ تقديم البحث 26/11/2017 | |||||
| تاريخ قبول البحث 26/11/2017 | |||||
| عدد صفحات البحث 14 | |||||
| Samia A. Haroun - مؤلف رئيسي | |||||
| Amr M. Mowafy | |||||
| Magdy M. El-shazly | |||||
| Mottia A. Sheir | |||||
| Keywords: Effective microorganisms Phaseolus vulgaris Wastewater Drains Growth parameters Phytohormones |
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| Abstract Water resources are one of the most worldwide problems and wastewater represents a challenge from the environmental aspects. The aim of this study is to elucidate the role of effective microorganisms (EM) on alleviating the effect of wastewater on the growth and metabolism of Phaseolus vulgaris plants. The data recorded at the flowering stage of Phaseolus vulgaris growth indicated that the growth parameters (the shoot length, number of nods, leaf area, the shoot fresh weight, shoot dry weight, moister content, leaf area ratio relative growth rate, net assimilation rat), photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids, total chlorophylls (a+b), chlorophyll a/b and consequently total pigment), carbohydrates fractions ( total soluble sugars, polysaccharides and total carbohydrates), heavy metals accumulation (cadmium, lead, copper), showed a general significant and non significant decreased at concentrations 50% &100% of wastewater (El-Serw and El-Tawela drains) with and without EM respectively. Meanwhile, protein and elements contents (nitrogen, phosphorus, potassium), showed in general a non significant increase at concentration 50% &100% of wastewater with and without EM of the tested drains. The determined plant growth regulators (gibberellins, cytokinins and total auxins), generally decreased significantly while, abscisic acid generally increased significantly by wastewater (El-Serw and El-Tawela drains) at concentrations of 50% and 100% with and without EM, of the two used drains. It is observable that the response in the represented parameters in the presence of EM was not as such in its absence, i.e. the presence of EM along with wastewater for irrigation led to nullify partially the harmful effect on these parameters of Phaseolus vulgaris plant. |
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| Vegetation-Soil Relationship Associated with Moringa peregrina (Forssk.) Fiori. Population in Egypt / | |||||
| تخصص البحث : Basic Sciences | |||||
| Mansoura Journal of Environmental Sciences - Egypt JOESE / Vol.46 No. 2 - June2017 | |||||
| تاريخ تقديم البحث 26/11/2017 | |||||
| تاريخ قبول البحث 26/11/2017 | |||||
| عدد صفحات البحث 14 | |||||
| Maimona, A. Kurd - مؤلف رئيسي | |||||
| Ahmed, A. El-Khouly | |||||
| Ramadan, A. Shawky | |||||
| Nessrien, M. Hamadallah | |||||
| Keywords: Moringa peregrina Vegetation South Sinai Red Sea coastal desert |
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| Abstract The vegetation analysis and species distribution at the present study, emphasizing that the environmental factors has an important effect on the plant communities. About 69 species belonging to 56 genera related to 27 families were observed in the studied localities, distributed as follow: South Sinai was represented by one location namely: Feiran Oasis, while the Red Sea coastal desert was represented by three locations namely: Shaieb El-Banat Mountain, El-Qusier Wadis and Hanquf Mountain. The mentioned localities dominated by M. peregrina. The floristic analysis recognized that, the most characteristic family was Asteraceae represented by 10 species (14.5%), followed by Fabaceae (8 species = 11.59%). The life forms obtained that; Chamaephytes 26 species (37.7%), followed by Therophytes 25 species (36.2%). Phanerophytes represented by 11 species (15.9%) of the flora were the most frequent, indicating a typical desert lifeform spectrum. Chorological analysis revealed the Mono-regional elements contain (40 species = 58%) of the total number of species. The Biregional elements contain 26 species (37.7%). The Pluriregional elements are the poorly category distinguished in the studied localities contain 3 species = (4.3%). After application of the TWINSPAN, 7 vegetation groups (I-VII) were represented the studied localities, all of them are dominated by M. peregrina, while the application of (CCA-biplot) indicates significant associations between the floristic composition of the studied area and the edaphic factors such as gravels, electrical conductivity, pH, calcium carbonate, cations and anions, also the altitude was most effective factor on the distribution of M. peregrina and the associated species in the studied localities. |
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| Measurement of some Tumor Markers in Ovarian cancer by Flow Cytometry / | |||||
| تخصص البحث : Basic Sciences | |||||
| Mansoura Journal of Environmental Sciences - Egypt JOESE / Vol.46 No. 2 - June2017 | |||||
| تاريخ تقديم البحث 26/11/2017 | |||||
| تاريخ قبول البحث 26/11/2017 | |||||
| عدد صفحات البحث 10 | |||||
| Magdy M. Youssef - مؤلف رئيسي | |||||
| Sameh R. Abd El-Aziz | |||||
| Mona S. Gouida | |||||
| Aya G. Elwan | |||||
| Keywords: Ovarian cancer Cancer antigen 125 Cell cycle |
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| Abstract Ovarian cancer is among the most lethal types of reproductive system tumors and a leading cause of cancer-related deaths in women globally cancer antigen125 (CA125) is the most widely used tumor marker in ovarian cancer. It is elevated in about 80% of women with epithelial with early stage of disease. Flow cytometric analysis of cell cycle measures the apoptotic changes in cells by staining them with DNA dyes. In this study, we aimed to measure level of CA125, liver function test and complete blood count by biochemical and haematological methods. Cell cycle is analyzed by flow cytometry in epithelial ovarian cancer and benign diseases. The results showed that, the level of CA 125 is significantly increased in malignant group than in normal and bengin groups (P = 0.001). The level of liver function which include serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), albumin and bilirubin is significantly increased in malignant group than in normal and benign group. The count of WBCS, RBCS and hemoglobin levels is decreased but that of platelets count is increased in malignant than in normal and benign groups. Sub-G1 apoptotic level is statistically significantly decreased in benign and malignant group than that in normal group (P = 0.001). Also, The data showed that S phase level had statistically significant increased in malignant group than that in the normal group (P= 0.001). This study explained the changes in sub-G1 apoptosis for normal and benign groups that was increased significantly than that in the malignant group level. |
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