عرض تفاصيل البحث
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Measurement of some Tumor Markers in Ovarian cancer by Flow Cytometry / | |||||
تخصص البحث : Basic Sciences | |||||
Mansoura Journal of Environmental Sciences - Egypt JOESE / Vol.46 No. 2 - June2017 | |||||
Magdy M. Youssef - مؤلف رئيسي | |||||
Sameh R. Abd El-Aziz | |||||
Mona S. Gouida | |||||
Aya G. Elwan | |||||
Keywords: Ovarian cancer Cancer antigen 125 Cell cycle |
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Abstract Ovarian cancer is among the most lethal types of reproductive system tumors and a leading cause of cancer-related deaths in women globally cancer antigen125 (CA125) is the most widely used tumor marker in ovarian cancer. It is elevated in about 80% of women with epithelial with early stage of disease. Flow cytometric analysis of cell cycle measures the apoptotic changes in cells by staining them with DNA dyes. In this study, we aimed to measure level of CA125, liver function test and complete blood count by biochemical and haematological methods. Cell cycle is analyzed by flow cytometry in epithelial ovarian cancer and benign diseases. The results showed that, the level of CA 125 is significantly increased in malignant group than in normal and bengin groups (P = 0.001). The level of liver function which include serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), albumin and bilirubin is significantly increased in malignant group than in normal and benign group. The count of WBCS, RBCS and hemoglobin levels is decreased but that of platelets count is increased in malignant than in normal and benign groups. Sub-G1 apoptotic level is statistically significantly decreased in benign and malignant group than that in normal group (P = 0.001). Also, The data showed that S phase level had statistically significant increased in malignant group than that in the normal group (P= 0.001). This study explained the changes in sub-G1 apoptosis for normal and benign groups that was increased significantly than that in the malignant group level. |
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